![]() The results showed that both Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total score ( P=0.043) and NRS score ( P=0.002) decreased more in EA group than those in SA group. Fecal microbial analyses by 16S ribosomal RNA gene sequencing were carried out after collecting stools at T 0 and T 8 weeks (Four samples with changed defecation habits were excluded). Participants in both groups received 24 sessions within eight weeks. Eight non-acupoints that were separated from conventional acupoints or meridians were used for the SA group. Five obligatory acupoints and three adjunct acupoints were punctured in the EA group. Sixty participants were allocated to either EA group (n=30) or sham acupuncture (SA) group (n=30). We conducted a study of 60 participants with KOA and 30 matched healthy controls (HCs). Herein, we aim to investigate the effect of electroacupuncture (EA) on gut microbiota in participants with KOA. 6Department of Orthopedics, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, ChinaĪ close relationship between knee osteoarthritis (KOA) and gut microbiota has recently been described.5Department of Acupuncture and Moxibustion, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.4Department of Acupuncture and Moxibustion, Guang’an Men Hospital, China Academy of Chinese Medical Sciences, Beijing, China.3Department of Acupuncture and Moxibustion, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.2National Institute of Traditional Chinese Medicine Constitution and Preventive Treatment of Diseases, Beijing University of Chinese Medicine, Beijing, China.1International Acupuncture and Moxibustion Innovation Institute, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.Tian-Qi Wang 1†, Ling-Ru Li 2†, Chun-Xia Tan 1, Jing-Wen Yang 1, Guang-Xia Shi 1, Li-Qiong Wang 1, Hui Hu 3, Zhi-Shun Liu 4, Jun Wang 5, Tong Wang 6, Yong Yuan 6, Wen-Rui Jia 1, Hua Li 3, Xin-Wei Wang 4, Bin Wu 5, Jian-Feng Tu 1* and Cun-Zhi Liu 1
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